Phenotypic and phylogenetic characterization of dominant culturable methanogens isolated from ricefield soils
Identifieur interne : 00C892 ( Main/Exploration ); précédent : 00C891; suivant : 00C893Phenotypic and phylogenetic characterization of dominant culturable methanogens isolated from ricefield soils
Auteurs : C. Joulian [France] ; B. Ollivier [France] ; B. K. C. Patel [Australie] ; P. A. Roger [France]Source :
- FEMS Microbiology Ecology [ 0168-6496 ] ; 1998.
Descripteurs français
- Pascal (Inist)
- Wicri :
English descriptors
- KwdEn :
- Acetotrophs, Anaerobic, Average similarity, Barkeri, Basal medium, Broad range, Bryantii, Camargue, Dominance, Dominant strains, Ecology, Energy source, Energy sources, Enumeration(counting), Fems, Fems microbiol, Fems microbiology ecology, Femsec, Formate, Formicicum, Genus, Genus methanobacterium, Genus methanoculleus, Hungate, Hungate tubes, Hydrogenotrophs, Irregular coccus, Japanese soils, Joulian, Major trophic groups, Marine sediments, Marisnigri, Mazei, Methane, Methanobacterium, Methanobacterium bryantii, Methanobacterium formicicum, Methanobacterium species, Methanobacterium strains, Methanoculleus, Methanoculleus marisnigri, Methanogen, Methanogenic, Methanogenic bacteria, Methanogenium, Methanol, Methanosarcina, Methanosarcina barkeri, Methanosarcina mazei, Microbiol, Microbiology, Oryza sativa, Paddy, Phenotype, Phenotypic, Phylogenetic, Phylogenetic analysis, Phylogeny, Population density, Population structure, Primer, Rrna, Rrna gene, Rrna sequences, Sarcinae, Selective media, Strain specificity.
- Teeft :
- Acetotrophs, Anaerobic, Average similarity, Barkeri, Basal medium, Broad range, Bryantii, Camargue, Dominant strains, Ecology, Energy source, Energy sources, Fems, Fems microbiol, Fems microbiology ecology, Femsec, Formate, Formicicum, Genus, Genus methanobacterium, Genus methanoculleus, Hungate, Hungate tubes, Hydrogenotrophs, Irregular coccus, Japanese soils, Joulian, Major trophic groups, Marine sediments, Marisnigri, Mazei, Methane, Methanobacterium, Methanobacterium bryantii, Methanobacterium formicicum, Methanobacterium species, Methanobacterium strains, Methanoculleus, Methanoculleus marisnigri, Methanogen, Methanogenic, Methanogenic bacteria, Methanogenium, Methanol, Methanosarcina, Methanosarcina barkeri, Methanosarcina mazei, Microbiol, Microbiology, Paddy, Phenotypic, Phylogenetic, Phylogenetic analysis, Primer, Rrna, Rrna gene, Rrna sequences, Sarcinae, Selective media.
Abstract
Abstract: Populations of the four major trophic groups of methanogens were enumerated by most probable numbers (MPN) on selective media in a sample of 13 soils representative of major types of rice soils. Dominant strains were isolated and their phenotypic and phylogenetic characteristics were studied. MPN counts ranged from 102 to 106 g−1 d.w. on H2, from <10 to 104 g−1 d.w. on acetate, from <10 to 105 on methanol, and from 50 to 106 on formate. In most soils, counts of hydrogenotrophs were higher than counts of acetotrophs, partly because acetotrophs were aggregated sarcinae difficult to separate into individual cells. Methylotrophs other than acetotrophic sarcinae were not recorded. In most soils, rods enumerated on formate were 5–400 times less abundant than those enumerated on H2, indicating that hydrogenotrophic-non-formatotrophic rods are probably dominant in ricefields. Dominant strains isolated comprised: 15 hydrogenotrophic-non-formatotrophic rods affiliated to Methanobacterium bryantii; three hydrogenotrophic-formatotrophic rods affiliated to Mb. formicicum; one hydrogenotrophic-formatotrophic rod not affiliated to a sequenced species; two sarcinae affiliated to Methanosarcina barkeri and Methanosarcina mazei; and one irregular coccus affiliated to Methanoculleus marisnigri – a species so far isolated from marine sediments only. Results from classical counts of methanogens and strains isolated from ricefields suggest the dominance of Methanobacterium spp. (mostly responsible for CH4 production from H2/CO2) and Methanosarcina spp. (mostly responsible for CH4 production from acetate) among culturable organisms. Both genera are probably ubiquitous. In particular, Mb. bryantii was isolated from 12 of the 13 soils.
Url:
DOI: 10.1016/S0168-6496(97)00090-1
Affiliations:
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sediments</term><term>Marisnigri</term><term>Mazei</term><term>Methane</term><term>Methanobacterium</term><term>Methanobacterium bryantii</term><term>Methanobacterium formicicum</term><term>Methanobacterium species</term><term>Methanobacterium strains</term><term>Methanoculleus</term><term>Methanoculleus marisnigri</term><term>Methanogen</term><term>Methanogenic</term><term>Methanogenic bacteria</term><term>Methanogenium</term><term>Methanol</term><term>Methanosarcina</term><term>Methanosarcina barkeri</term><term>Methanosarcina mazei</term><term>Microbiol</term><term>Microbiology</term><term>Oryza sativa</term><term>Paddy</term><term>Phenotype</term><term>Phenotypic</term><term>Phylogenetic</term><term>Phylogenetic analysis</term><term>Phylogeny</term><term>Population density</term><term>Population structure</term><term>Primer</term><term>Rrna</term><term>Rrna gene</term><term>Rrna sequences</term><term>Sarcinae</term><term>Selective media</term><term>Strain specificity</term></keywords><keywords scheme="Pascal" xml:lang="fr"><term>Bactérie méthanogène</term><term>Densité population</term><term>Dominance</term><term>Dénombrement</term><term>Methanobacterium</term><term>Oryza sativa</term><term>Phylogenèse</term><term>Phénotype</term><term>Sol rizière</term><term>Spécificité souche</term><term>Structure population</term></keywords><keywords scheme="Teeft" xml:lang="en"><term>Acetotrophs</term><term>Anaerobic</term><term>Average similarity</term><term>Barkeri</term><term>Basal medium</term><term>Broad range</term><term>Bryantii</term><term>Camargue</term><term>Dominant strains</term><term>Ecology</term><term>Energy source</term><term>Energy sources</term><term>Fems</term><term>Fems microbiol</term><term>Fems microbiology ecology</term><term>Femsec</term><term>Formate</term><term>Formicicum</term><term>Genus</term><term>Genus methanobacterium</term><term>Genus methanoculleus</term><term>Hungate</term><term>Hungate tubes</term><term>Hydrogenotrophs</term><term>Irregular coccus</term><term>Japanese soils</term><term>Joulian</term><term>Major trophic groups</term><term>Marine sediments</term><term>Marisnigri</term><term>Mazei</term><term>Methane</term><term>Methanobacterium</term><term>Methanobacterium bryantii</term><term>Methanobacterium formicicum</term><term>Methanobacterium species</term><term>Methanobacterium strains</term><term>Methanoculleus</term><term>Methanoculleus marisnigri</term><term>Methanogen</term><term>Methanogenic</term><term>Methanogenic bacteria</term><term>Methanogenium</term><term>Methanol</term><term>Methanosarcina</term><term>Methanosarcina barkeri</term><term>Methanosarcina mazei</term><term>Microbiol</term><term>Microbiology</term><term>Paddy</term><term>Phenotypic</term><term>Phylogenetic</term><term>Phylogenetic analysis</term><term>Primer</term><term>Rrna</term><term>Rrna gene</term><term>Rrna sequences</term><term>Sarcinae</term><term>Selective media</term></keywords><keywords scheme="Wicri" type="topic" xml:lang="fr"><term>écologie</term><term>Méthanol</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: Populations of the four major trophic groups of methanogens were enumerated by most probable numbers (MPN) on selective media in a sample of 13 soils representative of major types of rice soils. Dominant strains were isolated and their phenotypic and phylogenetic characteristics were studied. MPN counts ranged from 102 to 106 g−1 d.w. on H2, from <10 to 104 g−1 d.w. on acetate, from <10 to 105 on methanol, and from 50 to 106 on formate. In most soils, counts of hydrogenotrophs were higher than counts of acetotrophs, partly because acetotrophs were aggregated sarcinae difficult to separate into individual cells. Methylotrophs other than acetotrophic sarcinae were not recorded. In most soils, rods enumerated on formate were 5–400 times less abundant than those enumerated on H2, indicating that hydrogenotrophic-non-formatotrophic rods are probably dominant in ricefields. Dominant strains isolated comprised: 15 hydrogenotrophic-non-formatotrophic rods affiliated to Methanobacterium bryantii; three hydrogenotrophic-formatotrophic rods affiliated to Mb. formicicum; one hydrogenotrophic-formatotrophic rod not affiliated to a sequenced species; two sarcinae affiliated to Methanosarcina barkeri and Methanosarcina mazei; and one irregular coccus affiliated to Methanoculleus marisnigri – a species so far isolated from marine sediments only. Results from classical counts of methanogens and strains isolated from ricefields suggest the dominance of Methanobacterium spp. (mostly responsible for CH4 production from H2/CO2) and Methanosarcina spp. (mostly responsible for CH4 production from acetate) among culturable organisms. Both genera are probably ubiquitous. In particular, Mb. bryantii was isolated from 12 of the 13 soils.</div></front></TEI><affiliations><list><country><li>Australie</li><li>France</li></country><region><li>Provence-Alpes-Côte d'Azur</li></region><settlement><li>Marseille</li></settlement><orgName><li>Université de Provence</li></orgName></list><tree><country name="France"><region name="Provence-Alpes-Côte d'Azur"><name sortKey="Joulian, C" sort="Joulian, C" uniqKey="Joulian C" first="C." last="Joulian">C. Joulian</name></region><name sortKey="Ollivier, B" sort="Ollivier, B" uniqKey="Ollivier B" first="B." last="Ollivier">B. Ollivier</name><name sortKey="Roger, P A" sort="Roger, P A" uniqKey="Roger P" first="P. A." last="Roger">P. A. Roger</name></country><country name="Australie"><noRegion><name sortKey="Patel, B K C" sort="Patel, B K C" uniqKey="Patel B" first="B. K. C." last="Patel">B. K. C. Patel</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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